E-ISSN 2146-8362
 

Original Research 


Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers

Adeola Mofoluwake Ladeji, Kehinde Emmanuel Adebiyi, Akinwumi Komolafe, Olujide Oladele Soyele, Mofoluwaso Olajide, Henry Ademola Adeola.

Abstract
Background: Pleomorphic adenoma (PA) histopathologically represents a heterogeneous lesion with varying proportion of mesenchymal and epithelial tissue. Due to its heterogeneity, identifying various subtypes often presents a diagnostic dilemma. Hence the aim of this study is to determine and compare activities of salivary myoepithelial (myo) and basal reserve cells (BRC) within subtypes of salivary PA using p63 and alpha smooth muscle actin (α-SMA) immunohistochemical markers.
Method: In this retrospective study, 24 archival tissue blocks of PA (12 stroma-rich, 6 cell-rich and 6 classic subtypes) retrieved and subjected to immunohistochemistry using monoclonal p63 and α-SMA antibodies. Fraction of positive cells expressed as percentage of positive cells within each sample, Labeling Indices (LI) was computed.
Result: PA samples subjected to immunohistochemistry showed 100% p63 and 95.9% α-SMA immunoreactivity. Statistically significant difference (p=0.01) was observed with p63 Mean LI across PA subtypes. Overall, p63 LI (37.113.5) was higher than α-SMA LI (26.59.5). Staining pattern for both markers was similar within epithelial and stroma elements.
Conclusion: Disparity in Mean LI of the markers with higher index observed for p63, suggests additional activity of BRC, whose variable expression is associated with the differential ratio of epithelial to stroma components within PA subtypes.

Key words: Pleomorphic adenoma, p63, Alpha smooth muscle actin, labelling index


 
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Pubmed Style

Ladeji AM, Adebiyi KE, Komolafe A, Soyele OO, Olajide M, Adeola HA, . Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. J Interdiscip Histopathol. 2018; 6(2): 61-66. doi:10.5455/jihp.20181004012403


Web Style

Ladeji AM, Adebiyi KE, Komolafe A, Soyele OO, Olajide M, Adeola HA, . Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. http://www.ejmjih.com/?mno=11351 [Access: March 22, 2019]. doi:10.5455/jihp.20181004012403


AMA (American Medical Association) Style

Ladeji AM, Adebiyi KE, Komolafe A, Soyele OO, Olajide M, Adeola HA, . Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. J Interdiscip Histopathol. 2018; 6(2): 61-66. doi:10.5455/jihp.20181004012403



Vancouver/ICMJE Style

Ladeji AM, Adebiyi KE, Komolafe A, Soyele OO, Olajide M, Adeola HA, . Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. J Interdiscip Histopathol. (2018), [cited March 22, 2019]; 6(2): 61-66. doi:10.5455/jihp.20181004012403



Harvard Style

Ladeji, A. M., Adebiyi, . K. E., Komolafe, . A., Soyele, . O. O., Olajide, . M., Adeola, . H. A. & (2018) Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. J Interdiscip Histopathol, 6 (2), 61-66. doi:10.5455/jihp.20181004012403



Turabian Style

Ladeji, Adeola Mofoluwake, Kehinde Emmanuel Adebiyi, Akinwumi Komolafe, Olujide Oladele Soyele, Mofoluwaso Olajide, Henry Ademola Adeola, and . 2018. Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. Journal of Interdisciplinary Histopathology, 6 (2), 61-66. doi:10.5455/jihp.20181004012403



Chicago Style

Ladeji, Adeola Mofoluwake, Kehinde Emmanuel Adebiyi, Akinwumi Komolafe, Olujide Oladele Soyele, Mofoluwaso Olajide, Henry Ademola Adeola, and . "Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers." Journal of Interdisciplinary Histopathology 6 (2018), 61-66. doi:10.5455/jihp.20181004012403



MLA (The Modern Language Association) Style

Ladeji, Adeola Mofoluwake, Kehinde Emmanuel Adebiyi, Akinwumi Komolafe, Olujide Oladele Soyele, Mofoluwaso Olajide, Henry Ademola Adeola, and . "Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers." Journal of Interdisciplinary Histopathology 6.2 (2018), 61-66. Print. doi:10.5455/jihp.20181004012403



APA (American Psychological Association) Style

Ladeji, A. M., Adebiyi, . K. E., Komolafe, . A., Soyele, . O. O., Olajide, . M., Adeola, . H. A. & (2018) Immunohistochemical identification of salivary pleomorphic adenoma subtypes using p63 and alpha smooth muscle actin myoepithelial markers. Journal of Interdisciplinary Histopathology, 6 (2), 61-66. doi:10.5455/jihp.20181004012403





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