Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey
bees (Apis mellifera ligustica)

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano

2019, Vol: 7, Issue: 2

7 / 2Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Required files to be uploaded

Author Agreement Form

« Previous Article

Short Communication

J Interdiscip Histopathol. 2013; 1(3): 160-162

doi: 10.5455/jihp.20130128010436

Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano.

Cited by (1)

Abstract
Abstract
Objective: Chalkbrood is a fungal disease of honey bee brood caused by Ascosphaera apis. This disease is now found around the world and there are some indications that the incidence has increased in recent years. The diagnosis of Chalkbrood is based on the recognition of typical clinical symptoms and on the identification of the causative agent. Despite the numerous methods available for the detection of A. apis, the diagnosis of this pathology is not always easy. Methods: Between June and July 2011 an increase in mortality of honey bees was observed to some apiaries of the Caserta province (Campania region, southern Italy). Larvae (no. = 50) from brood samples without signs of disease and adult honey bees (no. = 50) from the same colonies were collected and submitted to our laboratory for routine histologic evaluation.Results and Conclusions: We demonstrate that histopathology is a sensitive and specific method for the detection and identification of Ascosphaera apis infection and has the additional advantage of being able to provide a diagnosis in larvae without signs of disease.

Key words: histopathological method, Ascosphaera apis, honey bee.

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Paola Maiolino Articles by Francesca Carella Articles by Giovanna De Leva Articles by Laura Rinaldi Articles by Giuseppe Cringoli Articles by Manuela Martano
	on Google
	on Google Scholar
	Article Statistics
	Viewed: 6223 Downloaded: 1052 Cited: 1

REFERENCES

Spiltoir CF. Life cycle of Ascosphaera apis (Pericystis apis). American Journal Botany 1955; 42: 501-508. http://dx.doi.org/10.2307/2438686

http://dx.doi.org/10.2307/2438686

Spiltoir CF, Olive LS. A re-classification of the genus Pericystis Betts. Mycologia 1955; 47: 238-244. http://dx.doi.org/10.2307/3755414

http://dx.doi.org/10.2307/3755414

Koening JP, Boush GM, Erickson EH. Isolation of the chalkbrood pathogen, Ascosphaera apis, from honeybee (Apis mellifera) surfaces, pollen loads and a water source. American Bee Journal 1987; 127: 581-583.

Hitchcock JD, Christensen M. Occurrence of Chalkbrood (Ascosphaera apis) in honeybees in the United States. Mycologia 1972; 64: 1193-1198.

http://dx.doi.org/10.2307/3758091

Kluser S, Peduzzi P. Global pollinator decline: a literature review. UNEP/GRID-Europe 2007; 5 (1): pp. 1–12.

Carrera P, Sommaragua A, Vailiti G. The development of Ascophaera apis within larvae of Apis mellifera ligustica. Journal of Apicultural Research 1987; 26: 59-63.

Skou JP. More details in support of the class Ascosphaeromycetes. Mycotaxon 1988; 31 (1): 191–198.

This Article Cited By the following articles

Histopathological findings of the midgut in European honey bee (Apis Mellifera L.) naturally infected by Nosema spp. Vet Med Anim Sci 2014; 2(1): 4.	1

How to Cite this Article

Pubmed Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Web Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). http://www.ejmjih.com/?mno=31740 [Access: February 15, 2020]. doi:10.5455/jihp.20130128010436

AMA (American Medical Association) Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Vancouver/ICMJE Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. (2013), [cited February 15, 2020]; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Harvard Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

Turabian Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. 2013. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

Chicago Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1 (2013), 160-162. doi:10.5455/jihp.20130128010436

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

Contact Information

Your comments are very important to us

How to cite this article

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access. The articles in Journal of Interdisciplinary Histopathology are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

Copyright © 2020 Journal of Interdisciplinary Histopathology All Rights Reserved. Subject to change without notice from or liability to Journal of Interdisciplinary Histopathology. For best results, please use Internet Explorer or Google Chrome	Contact Information Your comments are very important to us We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below. Email us at info[at]scopemed.com We will respond within 48 to 72 hours. Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.