Journal of Interdisciplinary Histopathology

2019, Vol: 7, Issue: 1

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J Interdiscip Histopathol. 2013; 1(3): 160-162

doi: 10.5455/jihp.20130128010436

Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano.

Cited by (1)

Abstract
Abstract
Objective: Chalkbrood is a fungal disease of honey bee brood caused by Ascosphaera apis. This disease is now found around the world and there are some indications that the incidence has increased in recent years. The diagnosis of Chalkbrood is based on the recognition of typical clinical symptoms and on the identification of the causative agent. Despite the numerous methods available for the detection of A. apis, the diagnosis of this pathology is not always easy. Methods: Between June and July 2011 an increase in mortality of honey bees was observed to some apiaries of the Caserta province (Campania region, southern Italy). Larvae (no. = 50) from brood samples without signs of disease and adult honey bees (no. = 50) from the same colonies were collected and submitted to our laboratory for routine histologic evaluation.Results and Conclusions: We demonstrate that histopathology is a sensitive and specific method for the detection and identification of Ascosphaera apis infection and has the additional advantage of being able to provide a diagnosis in larvae without signs of disease.

Key words: histopathological method, Ascosphaera apis, honey bee.

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How to Cite this Article

Pubmed Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Web Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). http://www.ejmjih.com/?mno=31740 [Access: April 18, 2019]. doi:10.5455/jihp.20130128010436

AMA (American Medical Association) Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Vancouver/ICMJE Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. (2013), [cited April 18, 2019]; 1(3): 160-162. doi:10.5455/jihp.20130128010436

Harvard Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

Turabian Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. 2013. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

Chicago Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1 (2013), 160-162. doi:10.5455/jihp.20130128010436

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436

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