E-ISSN 2146-8362
 

Original Research 


Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria

Jude Ogechukwu Okoye, Success Iwuanyanwu, Goodnews Ubong Nathaniel, Olusegun Agboola, Chisom Offor, John Kennedy Belonwu.

Abstract
Background: Aflatoxin B1 (AFB1), hepatitis B virus (HBV) and p53 mutation are major risk factors for liver cancer. However, the presence of AFB1 and expression of mutant p53 protein during active HBV replication (nuclear expression of Hepatitis B Core Antigen; HBcAg) and HBV clearance (nuclear expression of HBcAg) is still under-investigated in Africa.
Objectives: This study assessed the prevalence, extent of expression, interaction, and risk associated with single and binary expression of AFB1, HBcAg and mutant p53 (mtp53) in liver diseases in relation to age and sex.
Materials and Methods: This retrospective study included 14 and 74 cases of liver cancer (LC) and chronic hepatitis, respectively. Tissues were histochemically and immunohistochemically stained, scored and documented as positive (+) or negative (-) for antigen.
Result: In this study, the prevalence of AFB1, HBcAg, and mtp53 were 76.1%, 40.9%, and 61.4%, respectively. Higher prevalence and expression of AFB1 was observed in chronic hepatitis than in LC (p= 0.035 and 0.028, respectively). Higher prevalence of AFB1 and mtp53 and lower prevalence of HBcAg were observed in females than in males (p= 0.186, 0.0003 and 0.062, respectively). Cytoplasmic expression of HBcAg was higher in males (58 than in females were 58.6% (17/29) and 14.3% (1/7), respectively (p= 0.088). AFB1+ and HBcAg+ females were more and less likely to develop LC than males (OR: 3.00 and 0.39, 95%CI: 0.10-4.18 and 0.44-12.20, p= 0.012 and 0.003), respectively. AFB1+mtp53- (23.9%), AFB1+mtp53+ (52.3%), HBcAg+mtp53- (13.6%), HBcAg+mtp53+ (27.3%) and AFB1+HBcAg+ (34.1%) increased liver cancer risk (OR: 6.45, 21.50, 2.80, 0.78, and 5.11; 95%CI: 0.89-29.29, 0.95-43.86, 2.96-156.13, 0.60-13.01, and 0.14-4.25; p= 0.057, 0.002, 0.189, 0.772, and 0.067, respectively).
Conclusion: This study suggests that women are at a higher risk of AFB1 exposure and p53 mutation, but are at a lower risk of viral replication than men.

Key words: Liver cancer, Chronic hepatitis, Aflatoxin B1, Hepatitis B core Antigen, Mutant p53


 
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Pubmed Style

Okoye JO, Iwuanyanwu S, Nathaniel GU, Agboola O, Offor C, Belonwu JK. Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. J Interdiscip Histopathol. 2019; 7(1): 10-19. doi:10.5455/jihp.20190824114245


Web Style

Okoye JO, Iwuanyanwu S, Nathaniel GU, Agboola O, Offor C, Belonwu JK. Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. http://www.ejmjih.com/?mno=62606 [Access: February 15, 2020]. doi:10.5455/jihp.20190824114245


AMA (American Medical Association) Style

Okoye JO, Iwuanyanwu S, Nathaniel GU, Agboola O, Offor C, Belonwu JK. Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. J Interdiscip Histopathol. 2019; 7(1): 10-19. doi:10.5455/jihp.20190824114245



Vancouver/ICMJE Style

Okoye JO, Iwuanyanwu S, Nathaniel GU, Agboola O, Offor C, Belonwu JK. Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. J Interdiscip Histopathol. (2019), [cited February 15, 2020]; 7(1): 10-19. doi:10.5455/jihp.20190824114245



Harvard Style

Okoye, J. O., Iwuanyanwu, . S., Nathaniel, . G. U., Agboola, . O., Offor, . C. & Belonwu, . J. K. (2019) Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. J Interdiscip Histopathol, 7 (1), 10-19. doi:10.5455/jihp.20190824114245



Turabian Style

Okoye, Jude Ogechukwu, Success Iwuanyanwu, Goodnews Ubong Nathaniel, Olusegun Agboola, Chisom Offor, and John Kennedy Belonwu. 2019. Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. Journal of Interdisciplinary Histopathology, 7 (1), 10-19. doi:10.5455/jihp.20190824114245



Chicago Style

Okoye, Jude Ogechukwu, Success Iwuanyanwu, Goodnews Ubong Nathaniel, Olusegun Agboola, Chisom Offor, and John Kennedy Belonwu. "Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria." Journal of Interdisciplinary Histopathology 7 (2019), 10-19. doi:10.5455/jihp.20190824114245



MLA (The Modern Language Association) Style

Okoye, Jude Ogechukwu, Success Iwuanyanwu, Goodnews Ubong Nathaniel, Olusegun Agboola, Chisom Offor, and John Kennedy Belonwu. "Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria." Journal of Interdisciplinary Histopathology 7.1 (2019), 10-19. Print. doi:10.5455/jihp.20190824114245



APA (American Psychological Association) Style

Okoye, J. O., Iwuanyanwu, . S., Nathaniel, . G. U., Agboola, . O., Offor, . C. & Belonwu, . J. K. (2019) Mutant p53 protein is prevalent in Aflatoxin B1 than in Hepatitis B Core antigen associated chronic liver diseases in Southern Nigeria. Journal of Interdisciplinary Histopathology, 7 (1), 10-19. doi:10.5455/jihp.20190824114245





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